Buy Gram Stain Kit from TriStains brand | at Dawnscientific.com

Tristains gram staining kit consists of 4 bottles containing 250 mL of Gram Crystal Violet, Safranin, Gram Iodine and Gram Decolorizer each. Crystal Violet, a basic dye, is the primary stain taken up by all bacteria due to its ability to rapidly permeate the cell wall. It stains the protoplast of bacteria purple. The potassium-iodine mixture, in gram iodine, is the mordant which complexes with the primary stain in the cell. In gram-positive cells, the crystal violet-iodine complex is trapped in the cell due to a decrease in cell wall permeability caused by alcohol dehydration. Gram-positive cells are stained purple. In gram-negative cells, the complex is removed by the Decolorizer due to an increase in permeability caused by solubility of the lipids in alcohol. The counterstain used must be a contrasting color to the primary stain and, in this case, it is Safranin which stains gram-negative cells red. Not all products are available for sale in all territories.

Further more, Tristains Gram stain is a differential staining technique most widely applied in all microbiology disciplines laboratories. It is one of the most important criteria in any identification scheme for all types of bacterial isolates. Different mechanisms have been proposed to explain the gram reaction. There are many physiological differences between gram-positive and gram-negative cell walls. Ever since Christian Gram has discovered Gram staining, this process has been extensively investigated and redefined. In practice, a thin smear of bacterial cells is stained with crystal violet, then treated with an iodine containing mordant to increase the binding of primary stain. A decolourizing solution of alcohol or acetone is used to remove the crystal violet from cells which bind it weakly and then the counterstain (like safranin) is used to provide a colour contrast in those cells that are decolourized. Gram-positive bacteria have a thick mesh-like cell wall made of peptidoglycan (50–90% of cell envelope), and as a result are stained purple by crystal violet, whereas gram-negative bacteria have a thinner layer (10% of cell envelope), so do not retain the purple stain and are counter-stained pink by safranin. In a properly stained smear by gram staining procedure, the gram-positive bacteria appear blue to purple and gram-negative cells appear pink to red.

Overall, Tristains (Dawn scientific) Gram Stains kit ensure that you receive the finest quality dyes used in critical biological stains, offering both certifiable and BSC certified dyes. We Dawn Scientific, offers all types of quality stains Simple stains, Differential stains, Negative stains, stain kit as well as Structural/Special stains. We provide the stains in small packs as well as large quantities.

“TriStains”, Biological Stains that offer range of stains used in Histology, Cytology, Microbiology and Hematology laboratories.

TriStains meet the highest quality standards and give excellent color performance of desired components of cells and tissue in life science laboratories.

TriStains series products are carefully tested to ensure accurate, reliable, and reproducible results. Our products are available in different packaging sizes to allow you to get all types of stains & Indicators for your specific purposes from a single source.

In-addition Dawn Scientific also deals in numerous Laboratory Supplies, Chemicals, Equipment, Instruments, Reagents, Standard Solutions, Buffers, Biological Stains & Indicators and many more, for more information please visit our website www.dawnscientific.com you may also call on 1.-800-DAWN-SCI or email to sales@dawnscientific.com any Query/information we will be happy to help you.

Gram Stain Kit

Get High Quality Gram Stain kit -Pack of 4 of Tristains by Dawnscientific

High quality chemicals for all of your
laboratory requirements at lower price.

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