|Alternate Names||Basic Violet 10; Brilliant Pink B; Rhodamine O; Tetraethylrhodamine|
|Application||A useful fluorochrome for histology, FRET and mitochondrial probe|
|Supplemental Information||This is classified as a Dangerous Good for transport and may be subject to additional shipping charges|
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data (including water content).
Rhodamine B is a useful fluorochrome for histology due to its fluorescent properties in ultraviolet light. It ejects an electron onto TiO2, which is then scavenged by O2 forming the O2- radical anion. This anion ultimately becomes the OH radical. A pH-independent process degrades Rhodamine B in TiO2 dispersions containing no dodecylbenzenesulfonate. The compound has been incorporated in a FRET system based on lanthanide-doped nanoparticles for monitoring biological effects. Rhodamine B has also been used as a mitochondrial probe to study the energetic state of multi-drug resistant and sensitive cells where the mitochondrial Rhodamine B concentration was precisely determined with the loss of fluorescence in the presence of MTT Formazan (sc-215399), a fluorescence quencher. In addition, Rhodamine B has been observed to significantly reduce the number of human lip fibroblast cells in culture. Used for staining Negri bodies in tissue.
|Usage||Emission recorded at 545 nm|
|Solubility||Soluble in water (12 mg/ml), ethanol (70 mg/ml), acetone (slightly ), methyl cellosolve and alcohol.|
|Storage||Store at room temperature|
|Melting Point||210-211° C (lit.)(dec.)|
|Fluoresence||λex 554 nm, λem 627 nm in ethanol|
|Merck Index||14: 8183|