|Alternate Names||Basic Red 2; Safranin O; Cotton Red; Gossypimine;Safranin T;Safranin Y;Safranin A|
|Application||Safranin T is a biological stain used to stain Gram negative bacteria, chormosomes, mucin, cartilage and mast cells.|
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data (including water content).
Safranin T is a biological stain commonly used as a counter stain for gram-negative bacteria in the Gram stain procedure. Safranin T has been observed to bind to DNA at a pH 2.21. In the Flemming triple stain procedure Safranin T is used to stain for chromosomes. In late Palaeozoic pteridosperm cuticles Safranin T has shown increased contrast, emphasized cutinization differences, and enhanced the three-dimensional morphology of complex epidermal features when compared with other dyes. The compound has also been used with alcian blue in the histochemical study of the distribution and morphometric characteristics of mast cells. Safranin T has also been used to detect mucin and cartilage.
|Usage :||Gram stain procedure
• Prepare and heat-fix bacterial smears.
• Flood the slides crystal violet for one minute.
• Pour off excess dye and wash gently in tap water and drain the slide against a paper towel.
• Expose the smears to Gram’s iodine (sc-358803) for one minute by washing with iodine, then adding more iodine and leaving it on the smear until the minute is over.
• Wash with tap water and drain carefully. (Do not blot.)
• Wash with 95% alcohol for 30 seconds.
• Wash with tap water at the end of the 30 seconds to stop the decolorization. Drain.
• Counterstain with 25% Safranin T(sc-203758) for 30 seconds.
• Wash, drain, blot, and examine under oil.
|Solubility||Soluble in water (50 mg/ml at 20° C), and alcohol.|
|Storage||Store at room temperature|