Buy Safranine Solution (477-73-6) MF: C20H19ClN4 | MW: 350.84. Get High Quality Safranine Solution (477-73-6) from TriStians at Dawnscientific.com
Safranin Solution (Gram) is a reagent recommended for use in qualitative procedures to differentiate gram-negative from gram-positive organisms. The Gram staining allows a fast differentiation of bacteria in Gram-positive and Gram-negative. The mureine structure of the bacteria walls is the basis of the color affinity. Gram Safranin solution is used for contrast staining of bacterial species that did not retain their primary dye, i. e. Gram-negative bacteria. That enables differentiating the blue and purple-colored Gram-positive bacteria from the red-colored Gram-negative bacteria. Bacteria will be stained with Gram’s crystal violet solution – an aniline dye – in the first step. After the treatment with iod solution, a dye-iod complex will form. During the decolorizing step, this complex stays in the multilayer mureine structures of the Gram-positive bacteria, and they will appear blue. Gram-negative bacteria have a monolayer mureine structure only, the dye-iod complex does not stay bound to the cellwall, they will be decolorized. Gram-negative bacteria will be counterstained by safranin solution and appear orange.
“TriStains”, Biological Stains that offer range of stains used in Histology, Cytology, Microbiology and Hematology laboratories.
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Safranine Solution
Get High Quality Safranine Solution (477-73-6) from TriStians at Dawn Scientific. M.F: C20H19ClN4 | M.W.: 350.84, Shop now for reliable and Reproducible results
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