|Alternate Names||Sudan Black B is also known as Solvent Black 3, Fat Black HB, and CI 26150|
|Application||Sudan Black B is a fat-soluble diazo dye which has been used to stain phospholipids and intracellular lipids|
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data (including water content).
Sudan Black B is primarily used for staining of neutral triglycerides and lipids. Also a histochemical stain for chromosomes, Golgi apparatus, and leucocyte granules. Sudan Black B has the appearance of a dark brown to black powder with maximum absorption at 596-605 nm and it stains blue-black. Sudan dyes are a group of lipid soluble solvent dyes often called lysochromes, in structural classification they are diazo dyes. Sudan Black B is not as lipid-specific as some of the other Sudan dyes and may be useful to stain other materials. Sudan Black B is useful for detecting fats that are contaminated with oil and grease. Sudan black B is used in the study of haematological disorders as it will stain myeloblasts, but not lymphoblasts. Sudan Black B can also stain materials ionically; in this case some background staining is to be expected, and interpretation of sections stained with it must take this into account.
|Solubility||Soluble in oils, fats, warm petrolatum, paraffin, phenol, ethanol (10 mg/mL), acetone, benzene, toluene, and hydrocarbon. Insoluble in water.|
|Storage||Store at 4° C|
|Melting Point||120-124° C (lit.)|
|pK Values :||pKb: 10.88|