|Alternate Names||Solvent Red 23; CI 26100|
|Application||Sudan III is a lysochrome (fat soluble dye) predominantly used for staining triglycerides in animal tissues (frozen sections)|
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data (including water content).
Sudan III is predominantly used for staining triglycerides in animal tissues (frozen sections). Sudan dyes are a group of lipid soluble solvent dyes often called lysochromes, in structural classification they are diazo dyes. With the use of certain solvents, may also be used to stain some protein bound lipids in paraffin sections. Sudan I (sc-215922), Sudan III (sc-203761), and Sudan IV (sc-203762) have been classified as category 3 carcinogens by the International Agency for Research on Cancer.
|Usage||• A saturated solution in isopropanol is prepared for use as a fat stain in animal tissue. Counterstain for 5 minutes in acid alum hematoxylin (approx. 0.1%). Fats will appear yellow orange in color and the nuclei will appear blue. The cytoplasm will appear green.
• Since lysochromes are soluble in non polar solvents, including xylene and toluene, and also in ethanol, these fluids must not be put on to sections after fats have been stained or the dye will be dissolved out; therefore resinous mounting media are not recommended. We recommend mounting sections with Crystal Mounting Medium; sc-24943.
|Solubility||Soluble in ether, acetone, chloroform, benzene, ethanol (slightly soluble), DMSO, Hot glacial acetic acid (Slightly), hot glycerol, and toluene. Insoluble in water, and Lye.|
|Storage||Store at room temperature|
|Melting Point||199° C (dec.)|
|Boiling Point||~584.6° C at 760 mmHg (Predicted)|
|Density||~1.2 g/cm3 (Predicted)|
|Refractive Index||n20D 1.66 (Predicted)|
|pK Values||pKa: 7.51|