|Alternate Names||Direct Blue 14, Dianil blue, Diaminine Blue|
|Application||Trypan Blue is a viability dye for quantifying live versus dead cells|
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data (including water content).
Trypan Blue is an azo based, hydrophilic, tetrasulfonated blue acid dye widely utilized for assessing cell viability. As an easy method to determining the viability of cells, Trypan Blue will stain dead cells with permeable membranes blue, while the dye is excluded by most living cells and their intact membranes thereby allowing visual determination of living versus dead cells. Although Trypan Blue is predominately used for assessing cell viability of cultured cells, other applications for this dye have been reported. In rat abdominal organ slices Trypan Blue has been utilized to determine cell viability as well as islet cell cluster cell viability from caprine pancreatic samples.
|Solubility||Soluble in water (10 mg/ml at 25° C), ethanol (0.6 mg/ml), and EGME (20 mg/ml).|
|Storage||Store at 4° C|
|Melting Point||>300° C (lit.)|
|IC50||SH-PTP2: IC50 = 3.9 µM (human); Protein-tyrosine phosphatase 1: IC50 = 7.4 µM (Saccharomyces cerevisiae); P2Y2: IC50 = 17.1 µM (human)|
|pK Values||pKa: -1.53 (Predicted), pKb: 2.39 (Predicted)|
|Merck Index||14: 9792|
|SMILES||CC1=C(C=CC(=C1)C2=CC(=C(C=C2)NN=C3C(=CC4=CC (=CC(=C4C3=O)N)S(=O)(=O)[O-])S(=O)(=O)[O-])C)NN=C5C (=CC6=CC(=CC(=C6C5=O)N)S(=O)(=O)[O-]) S(=O)(=O)[O-].[Na+].[Na+].[Na+].[Na+]|