


Formamide, Part Number 221198-1L-SA Buy now from Sigma-Aldrich at Dawnscientific.
Sigma-Aldrich Formamide ACS Reagent Grade delivers ≥99.5% purity, meeting and exceeding the rigorous standards of the American Chemical Society for reliable laboratory performance. Widely used in PCR amplification, nucleic acid hybridization, and palladium-catalyzed synthesis, this high-purity formamide is suitable for routine chemical synthesis, drying, purification, and critical labware cleaning. Available at Dawn Scientific for research, pharmaceutical, and molecular biology laboratories across the USA.
Key Features
Specifications Table
| Parameter | Details |
|---|---|
| Supplier | Sigma-Aldrich |
| Product Name | Formamide |
| Grade | ACS Reagent |
| Assay | ≥99.5% |
| Form | Liquid |
| Boiling Point | 210 °C (lit.) |
| Melting Point | 2–3 °C (lit.) |
| Freezing Point | 2.0–3.0 °C |
| Density | 1.134 g/mL at 25 °C (lit.) |
| Parameter | Details |
|---|---|
| Refractive Index | n20/D 1.447 (lit.) |
| Vapor Pressure | 0.08 mmHg at 20 °C / 30 mmHg at 129 °C |
| Vapor Density | 1.55 (vs air) |
| Autoignition Temp. | 932 °F |
| pH | 4–10 (20 °C, 200 g/L) |
| Color | APHA ≤10 |
| Technique(s) | PCR, chemical synthesis, hybridization |
| SMILES | NC=O |
| InChI Key | ZHNUHDYFZUAESO-UHFFFAOYSA-N |
Applications & Uses
Sigma-Aldrich Formamide ACS Reagent Grade is widely used across molecular biology, research, and industrial laboratory workflows, including:
Why Buy from Dawn Scientific?
Dawn Scientific is built on a commitment to certified quality and dependable supply. When you order through Dawn Scientific, you receive:
In addition to our specialty product lines, Dawn Scientific offers a comprehensive range of laboratory supplies and scientific products, including laboratory chemicals, reagents, analytical standards, solutions, buffers, biological stains, indicators, laboratory equipment, instruments, consumables, and much more.
For complete product information, technical resources, and our full catalog, please browse other categories on our website ( www.dawnscientific.com.)
If you have any questions or would like assistance selecting the right products for your application, our knowledgeable team is ready to help. Contact us at 1-800-DAWN-SCI or email sales@dawnscientific.com. We look forward to serving your laboratory and research needs.
Frequently Asked Questions (FAQs)
Sigma-Aldrich Formamide ACS Reagent Grade has a minimum assay purity of ≥99.5%, meeting or exceeding the rigorous standards of the American Chemical Society for reliable laboratory performance.
The addition of formamide may enhance the specificity and efficiency of PCR amplification, making it a valuable additive for challenging PCR reactions requiring improved accuracy.
Formamide destabilizes nucleic acid duplexes and is typically used at a concentration of 50% in hybridization protocols that require lower hybridization temperatures.
This formamide meets or exceeds the stringent standards set by the American Chemical Society, ensuring consistent, high-quality, and reproducible results suitable for research and publication.
Sigma-Aldrich Formamide ACS Reagent Grade is available for purchase online through Dawn Scientific, an authorized supplier offering genuine products with fast, reliable delivery across the USA.

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