Hematoxylin C.I. 75290 CAS: 517-28-2 | MF: – | MW: 302.28, Buy Histological Stains & Dyes for Histology, Cytology, Microbiology, Hematology & Biology Lab from TriStains at Dawnscientific.
Hematoxylin, also known as Hematoxylin cryst. (C.I. 75290) (CAS No. 517-28-2), is a natural dye derived from the heartwood of the logwood tree (Haematoxylum campechianum). When combined with a mordant, such as aluminum, and oxidized to hematein, it becomes an effective staining agent for biological tissues. Its chemical formula is C16H14O6. Hematoxylin crystals are used as a dye for the specific staining of DNA in the nuclei. Widely utilized in histology and cytology, hematoxylin selectively stains cell nuclei, providing excellent contrast against the background. The stained nuclei appear blue, dark violet to black.
Applications of Hematoxylin C.I. 75290
- Selective Nucleus Staining: Hematoxylin staining, having selective application, involves its use in nurturing the cell associated with DNA. This is an essential part of histology and cytology because it facilitates the examination of tissues, which is enhanced by contrast between the various components.
- Histopathology Staining: In Histopathology, where Hematoxylin is intensively used alongside Eosin in what is known as ‘Hematoxylin and Eosin’ (H&E) stain. One of its uses includes the staining of the nuclear apparatus of a cell using Hematoxylin while the cytoplasm and all other extracellular substances are stained using Eosin.
- Molecular and Cell Biology Studies: Hematoxylin is utilized in molecular biology and cell biology for the staining of non-cytoplasmic cell nuclei as well as gene expression, cell division, and other cellular activity processes.
Key Features of Hematoxylin C.I. 75290
- Very Contrasting and Specific Staining: Hematoxylin gives sharp coloration of cell nuclei, providing distinction between other fine details inside the cell and nuclear structure, which is very important.
- Flexible and Strong: Almost all tissue types can be double-stained using different Hematoxylin stains. Hematoxylin is one of the common stains used in both specialized and routine diagnostic work.
- Budget-Friendly: Compared to other dyes, hematoxylin is a relatively inexpensive stain, making it cost-effective for laboratories and research institutions.
- Consistent and reliable: Hematoxylin provides reliable results with reproducibility because it ensures repeated staining yields consistent outcomes, which is necessary for proper analysis.
General Description
Hematoxylin C.I. 75290 (CAS No. 517-28-2) is a natural dye that is obtained from the heartwood of the logwood tree (Haematoxylum campechianum). One of its most important uses is as a powerful stain in histology, cytology, and biological research, as it selectively stains the cell nuclei. Hematoxylin has a molecular formula of C16H14O6 and a molecular weight (MW) of 302.28. Hematoxylin serves as an excellent stain for biotechnological tissues, mainly those which contain DNA in their nuclei, after being oxidized and converted to hematein.
If you are interested in buying Hematoxylin C.I. 75290 (CAS No. 517-28-2), these stains and dyes, Dawn Scientific is a great option to consider. Since we deal with a varied store of stains and dyes used for histology and cytology-related fields, we guarantee that hemotoxylin for use in staining will be available for your laboratory’s needs. Our company aims to propel biological science, research, and even educational work by offering high-grade dyes and reagents needed in those fields.
We recommend visiting or reaching out to our customer service team to get the latest pricing and information related to bulk orders on Hematoxylin C.I. 75290 (CAS No. 517-28-2). We ensure customer satisfaction by providing support as well as offering competitive pricing.
About Brand
“TriStains”, Histological Stains/ Biological Stains that offer a range of stains used in Histology, Cytology, Microbiology, and Hematology laboratories.
TriStains meet the highest quality standards and give excellent color performance of desired components of cells and tissue in life science laboratories.
TriStains series products are carefully tested to ensure accurate, reliable, and reproducible results. Our products are available in different packaging sizes to allow you to get all types of stains & Indicators for your specific purposes from a single source.
Why Dawn Scientific?
In addition to our specialty product lines, Dawn Scientific offers a comprehensive range of laboratory supplies and scientific products, including laboratory chemicals, reagents, analytical standards, solutions, buffers, biological stains, indicators, laboratory equipment, instruments, consumables, and much more.
For complete product information, technical resources, and our full catalog, please browse other categories on our website dawnscientific.com.
If you have any questions or would like assistance selecting the right products for your application, our knowledgeable team is ready to help. Contact us at 1-800-DAWN-SCI (1-800-329-6724) or email sales@dawnscientific.com. We look forward to serving your laboratory and research needs.
Frequently Asked Questions (FAQs)
TriStains Hematoxylin C.I. 75290 is widely used as a nuclear stain in histology, cytology, hematology, and pathology laboratories, particularly in Hematoxylin & Eosin (H&E) staining procedures.
TriStains Hematoxylin C.I. 75290 is commonly available in:
- • 25 g
- • 100 g
These pack sizes are suitable for routine laboratory, research, and diagnostic applications.
No, TriStains Hematoxylin C.I. 75290 is generally supplied as a high-purity crystalline powder for preparation of customized hematoxylin staining solutions such as Mayer’s, Harris, Gill, and Delafield formulations.
TriStains Hematoxylin C.I. 75290 is manufactured using high-purity staining-grade material. Industry reference standards for microscopy-grade hematoxylin commonly specify dye content of ?60% spectrophotometric assay for reliable staining performance.
It provides:
- • Strong nuclear staining intensity
- • Excellent chromatin detail visualization
- • High tissue contrast
- • Consistent batch-to-batch reproducibility
making it suitable for routine and advanced histological applications.
When used with appropriate mordants, it produces dark blue, blue-violet, or black nuclear staining, allowing clear visualization of cell nuclei and tissue morphology.
Yes, Hematoxylin C.I. 75290 is one of the most widely used dyes for Hematoxylin & Eosin (H&E) staining, providing sharp nuclear contrast against eosin-stained cytoplasmic structures.
It is widely used in:
- • Histology laboratories
- • Cytology studies
- • Tissue diagnostics
- • Pathology laboratories
- • Research and educational institutions
Yes, TriStains products are manufactured with strict quality control standards to ensure uniform staining behavior, reliable color development, and reproducible laboratory results.
TriStains Hematoxylin C.I. 75290 offers high-purity staining quality, dependable nuclear contrast, multiple packaging options, and consistent laboratory performance, making it suitable for histology, cytology, pathology, and research applications.








Reviews (0)
Please Login